产气荚膜梭菌四环素-氯霉素抗性质粒pIP401的物理特性

Physical characterization of the Clostridium perfringens tetracycline-chloramphenicol resistance plasmid pIP401.

作者信息

Magot M

出版信息

Ann Microbiol (Paris). 1984 Nov-Dec;135B(3):269-82. doi: 10.1016/s0769-2609(84)80094-0.

Abstract

Four restriction endonucleases were used to construct a physical map of the tetracycline-chloramphenicol resistance plasmid pIP401, which had been isolated from Clostridium perfringens. Twenty-seven restriction sites were placed within the 52-kb map, including 1 site for AvaI, 2 sites for KpnI, 10 sites for EcoRI and 14 sites for PstI. The loss of chloramphenicol resistance in the derived pIP406 plasmid was associated with a deletion of a 6.2-kb DNA segment located in a 10.55 EcoRI-PstI fragment. Two 1.4-kb inverted repeats were also characterized in both pIP401 and pIP406.

摘要

使用四种限制性内切核酸酶构建了从产气荚膜梭菌分离出的四环素-氯霉素抗性质粒pIP401的物理图谱。在52 kb的图谱中确定了27个限制性位点，包括1个AvaI位点、2个KpnI位点、10个EcoRI位点和14个PstI位点。衍生质粒pIP406中氯霉素抗性的丧失与位于10.55 EcoRI-PstI片段中的一段6.2 kb DNA片段的缺失有关。在pIP401和pIP406中还鉴定出两个1.4 kb的反向重复序列。