Wolf H, Haus M, Leser U, Modrow S, Motz M, Gu S Y, Falser N, Bandlow I, Richter W, Pathmanathan R
IARC Sci Publ. 1984(63):373-91.
Nucleic acid hybridization is widely used for scientific applications in specialized laboratories. This paper describes hybridization probes that can be prepared with less specialized equipment. A new indirect 'sandwich' hybridization test is described which allows the use of only one universally usable labelled probe for hybridization tests with specificities for various sequences. The use of different labels and hybridization techniques is also discussed and critically compared. For in situ hybridization, the usability of fixed and embedded materials is tested and evaluated.
