Pereira R, Tolosa de Talamoni N, Asteggiano C, Cañas F
Acta Physiol Pharmacol Latinoam. 1984;34(4):419-26.
The effect of diet phosphate content and cholecalciferol on intestinal phosphate secretion and absorption was investigated in rachitic chicks. Phosphate absorption was determined by the in situ ligated loop technique. Phosphate secretion was estimated by a method proposed by the authors. Hydroxyapatite, placed in the lumen of a ligated loop, acts as trapping agent of 32P leaving the intestinal tissue. The fraction of rapid exchangeability of tissue phosphate was taken as the precursor pool of secreted phosphate. Control chicks fed diets containing 0.3% P (group 1) or 1.0% (group 2) showed similar Pi absorption; the secretion was larger for group 2. After cholecalciferol treatment for 2 or 4 consecutive days an increment of Pi absorption with simultaneous reduction of Pi secretion was evident for both groups of animals. Chicks treated for 7 days gave values similar to those of controls. It is concluded that the regulation of intestinal phosphate absorption and secretion could be one important mean of homeostatic control. Intestinal phosphate movement is adapted to dietary phosphate and is partially independent of cholecalciferol.
在患佝偻病的雏鸡中研究了日粮磷含量和胆钙化醇对肠道磷分泌与吸收的影响。采用原位结扎肠袢技术测定磷吸收。磷分泌采用作者提出的方法估算。置于结扎肠袢肠腔内的羟基磷灰石用作离开肠组织的³²P的捕获剂。组织磷的快速交换部分被视为分泌磷的前体池。饲喂含0.3%磷日粮的对照雏鸡（第1组）或1.0%磷日粮的对照雏鸡（第2组）表现出相似的磷吸收；第2组的分泌量更大。连续2天或4天给予胆钙化醇处理后，两组动物的磷吸收均增加，同时磷分泌减少。处理7天的雏鸡得到的值与对照组相似。得出的结论是，肠道磷吸收和分泌的调节可能是体内稳态控制的一个重要手段。肠道磷的移动适应日粮磷，且部分独立于胆钙化醇。
