鉴定单纯疱疹病毒1型的一种基因功能，该功能对于在转化的仓鼠细胞中扩增猿猴病毒40 DNA序列至关重要。

Identification of a gene function of herpes simplex virus type 1 essential for amplification of simian virus 40 DNA sequences in transformed hamster cells.

作者信息

Matz B, Schlehofer J R, zur Hausen H

出版信息

Virology. 1984 Apr 30;134(2):328-37. doi: 10.1016/0042-6822(84)90301-5.

Abstract

Infection with herpes simplex viruses (HSV) lead to a significant increase of the simian virus 40 (SV40) DNA content in the SV40-transformed hamster cell lines CO631 and Elona. Analysis of this gene-amplifying activity revealed (i) that it cosedimented with infectious herpesvirions in sucrose density gradients, (ii) that it was abolished by anti-HSV antibodies or (iii) by antiviral drugs acting on the HSV-induced DNA polymerase; and analysis of temperature-sensitive mutants showed that this DNA polymerase was an essential component of HSV-induced, gene-amplifying activity in SV40-transformed hamster cells.

摘要

单纯疱疹病毒（HSV）感染会导致猿猴病毒40（SV40）转化的仓鼠细胞系CO631和埃洛娜中SV40 DNA含量显著增加。对这种基因扩增活性的分析表明：（i）它在蔗糖密度梯度中与感染性疱疹病毒粒子共沉降；（ii）抗HSV抗体可使其失活；（iii）作用于HSV诱导的DNA聚合酶的抗病毒药物也可使其失活；对温度敏感突变体的分析表明，这种DNA聚合酶是HSV诱导的、SV40转化的仓鼠细胞中基因扩增活性的必需成分。