Macnab J C, Adams R L, Rinaldi A, Orr A, Clark L
MRC Institute of Virology, Glasgow, United Kingdom.
Mol Cell Biol. 1988 Apr;8(4):1443-8. doi: 10.1128/mcb.8.4.1443-1448.1988.
Infection of rat embryo cells with herpes simplex virus type 2 caused undermethylation of host cell DNA synthesized during infection. DNA made prior to infection was not demethylated, but some of its degradation products, including methyl dCMP, were incorporated into viral DNA. The use of mutant virus showed that some viral DNA synthesis appears to be required for the inhibition of methylation. Inhibition of methylation cannot be explained by an absence of DNA methyltransferase as the activity of this enzyme did not change during the early period of infection. Inhibition of host cell DNA methylation may be an important step in the transformation of cells by herpesviruses, and various transformed cell lines tested showed reduced levels of DNA methylation.
用2型单纯疱疹病毒感染大鼠胚胎细胞会导致感染期间合成的宿主细胞DNA发生甲基化不足。感染前合成的DNA未发生去甲基化，但其一些降解产物，包括甲基脱氧胞苷单磷酸，被整合到病毒DNA中。使用突变病毒表明，抑制甲基化似乎需要一些病毒DNA的合成。甲基化的抑制不能用DNA甲基转移酶的缺失来解释，因为该酶的活性在感染早期并未改变。宿主细胞DNA甲基化的抑制可能是疱疹病毒使细胞发生转化的一个重要步骤，并且测试的各种转化细胞系显示出DNA甲基化水平降低。
