Narula S S, Dhingra M M
Chemical Physics Group, Tata Institute of Fundamental Research, Bombay, India.
J Biomol Struct Dyn. 1984 Aug;2(1):191-21. doi: 10.1080/07391102.1984.10507557.
The self-association of puromycin has been studied using proton magnetic resonance spectroscopy. The concentration, temperature and pH dependence studies of the proton chemical shifts of the adenine protons indicate that puromycin in aqueous solution at pD 7.4 self associates predominantly through adenine-adenine interaction. At this pD, the amino group of the aminoacyl segment of puromycin has been demonstrated to exist in a equilibrium blend of protonated and non-protonated forms. At pD 2.6, PM is found to exist predominantly in the monomeric from in which the methyl groups of the 6N-dimethyladenine are found to be non-equivalent due to hindered rotation about the C6-N6 bond.
已使用质子磁共振波谱法研究了嘌呤霉素的自缔合。对腺嘌呤质子的质子化学位移进行的浓度、温度和pH依赖性研究表明，在pD 7.4的水溶液中，嘌呤霉素主要通过腺嘌呤-腺嘌呤相互作用进行自缔合。在该pD下，已证明嘌呤霉素氨酰基片段的氨基以质子化和非质子化形式的平衡混合物存在。在pD 2.6时，发现嘌呤霉素主要以单体形式存在，其中6N-二甲基腺嘌呤的甲基由于围绕C6-N6键的旋转受阻而不等效。
