Vahtera E, Hamberg U
Biochem J. 1976 Aug 1;157(2):521-4. doi: 10.1042/bj1570521.
Pancreatic and urinary kallikreins failed to form the typical serine proteinase complex with alpha2M (alpha2-macroglobulin). Studies were performed to compare this with the binding of trypsin to alpha2M at various molar binding ratios, with the use of Sephadex G-200 gel filtration to separate free and alpha2M-bound enzyme fractions. The subunit conversion was totally absent with pancreatic kallikrein from lhich traces of a binding proteinase had been removed. The lack of binding is believed to be the result of the restricted specificity of the kallikreins.
胰腺激肽释放酶和尿激肽释放酶无法与α2M（α2-巨球蛋白）形成典型的丝氨酸蛋白酶复合物。进行了多项研究，以不同的摩尔结合比将胰蛋白酶与α2M的结合与之进行比较，使用Sephadex G-200凝胶过滤来分离游离的和与α2M结合的酶组分。去除了痕量结合蛋白酶的胰腺激肽释放酶完全不存在亚基转化。据信，结合的缺乏是激肽释放酶特异性受限的结果。
