α1-蛋白酶抑制剂对人组织激肽释放酶的抑制作用。

Inhibition of human tissue kallikrein by alpha 1-proteinase inhibitor.

作者信息

Hirano K, Okumura Y, Hayakawa S, Adachi T, Sugiura M

出版信息

Hoppe Seylers Z Physiol Chem. 1984 Jan;365(1):27-32. doi: 10.1515/bchm2.1984.365.1.27.

DOI:10.1515/bchm2.1984.365.1.27

PMID:6201429

Abstract

Tissue kallikreins, human urinary and pancreatic kallikrein, were inhibited by alpha 1-proteinase inhibitor, but the other inhibitors in plasma could not inhibit kallikrein activity towards a synthetic substrate. The kallikrein-inhibitor complex was identified by Sephadex G-150 gel filtration. The inhibition rate of urinary kallikrein by alpha 1-proteinase inhibitor was smaller than that of pancreatic kallikrein, and a less acidic subform of kallikrein was inhibited more rapidly than the more acidic one. Moreover, asialo-kallikrein was inhibited more rapidly than native kallikrein.

摘要

组织激肽释放酶、人尿激肽释放酶和胰激肽释放酶可被α1-蛋白酶抑制剂抑制，但血浆中的其他抑制剂不能抑制激肽释放酶对合成底物的活性。通过葡聚糖凝胶G-150凝胶过滤鉴定激肽释放酶-抑制剂复合物。α1-蛋白酶抑制剂对尿激肽释放酶的抑制率低于对胰激肽释放酶的抑制率，且酸性较弱的激肽释放酶亚型比酸性较强的亚型被抑制得更快。此外，去唾液酸激肽释放酶比天然激肽释放酶被抑制得更快。