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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Borucka-Mankiewicz M, Szarkowski J W

Acta Biochim Pol. 1977;24(4):289-99.

An endonuclease has been isolated from the nuclei of rye (Secale cereale L) germ and partially purified. The enzyme shows optimum activity over the pH range 5.4-7.4 towards both DNA and RNA, and has no phosphomonoesterase or phosphodiesterase activity. 2. DNA is degraded by the rye germ nuclease to oligonucleotides of similar size, and RNA to oligonucleotides and mononucleotides containing a C-terminal 5'-phosphate group. 3. The rate of hydrolysis of nuclear acids by the enzyme decreases in the following order: native DNA greater than denatured DNA greater than RNA. Synthetic polynucleotides are hydrolysed at a rate decreasing in the order: poly(A) greater than poly(U) greater than poly(C) greater than poly(G).

从黑麦（Secale cereale L）胚细胞核中分离出一种核酸内切酶，并进行了部分纯化。该酶在pH 5.4 - 7.4范围内对DNA和RNA均表现出最佳活性，且无磷酸单酯酶或磷酸二酯酶活性。2. 黑麦胚核酸酶将DNA降解为大小相似的寡核苷酸，将RNA降解为寡核苷酸和含有C端5'-磷酸基团的单核苷酸。3. 该酶对核酸的水解速率按以下顺序降低：天然DNA＞变性DNA＞RNA。合成多核苷酸的水解速率按以下顺序降低：聚（A）＞聚（U）＞聚（C）＞聚（G）。

Borucka-Mankiewicz M, Szarkowski J W

Acta Biochim Pol. 1977;24(4):289-99.

An endonuclease has been isolated from the nuclei of rye (Secale cereale L) germ and partially purified. The enzyme shows optimum activity over the pH range 5.4-7.4 towards both DNA and RNA, and has no phosphomonoesterase or phosphodiesterase activity. 2. DNA is degraded by the rye germ nuclease to oligonucleotides of similar size, and RNA to oligonucleotides and mononucleotides containing a C-terminal 5'-phosphate group. 3. The rate of hydrolysis of nuclear acids by the enzyme decreases in the following order: native DNA greater than denatured DNA greater than RNA. Synthetic polynucleotides are hydrolysed at a rate decreasing in the order: poly(A) greater than poly(U) greater than poly(C) greater than poly(G).

从黑麦（Secale cereale L）胚细胞核中分离出一种核酸内切酶，并进行了部分纯化。该酶在pH 5.4 - 7.4范围内对DNA和RNA均表现出最佳活性，且无磷酸单酯酶或磷酸二酯酶活性。2. 黑麦胚核酸酶将DNA降解为大小相似的寡核苷酸，将RNA降解为寡核苷酸和含有C端5'-磷酸基团的单核苷酸。3. 该酶对核酸的水解速率按以下顺序降低：天然DNA＞变性DNA＞RNA。合成多核苷酸的水解速率按以下顺序降低：聚（A）＞聚（U）＞聚（C）＞聚（G）。