Sykes A R, Coop R L, Robinson M G
Res Vet Sci. 1980 Jan;28(1):71-5.
The effect of low grade chronic Fasciola hepatica infection on the concentration of plasma glutamate dehydrogenase (GD), gamma-glutamyl transpeptidase (gamma-GT) and aspartate aminotransferase (AST) was investigated in sheep dosed daily with three (AL3), eight (AL8) or 14 (AL14) metacercariae over 22 weeks or given a single dose of 200 metacercariae. Significant increases in plasma GD activity first occurred after nine, 12 and 23 weeks and in gamma-GT activity after 12, 24 and 32 weeks for groups AL14, AL8 and AL3 respectively. Changes in AST activity were not as clearly related to dose level. In sheep with single infection, both GD and gamma-GT were capable of detecting liver damage resulting from the migration of 10 or more flukes. Plasma GD and gamma-GT activities are more sensitive indicators of liver cell damage in chronic subclinical fascioliasis than AST activity and gamma-GT may be more suitable as a diagnostic aid on account of its greater stability.
在22周内每天给绵羊分别投喂三只（AL3组）、八只（AL8组）或十四只（AL14组）肝片吸虫囊蚴，或单次投喂200只囊蚴，研究轻度慢性肝片吸虫感染对血浆谷氨酸脱氢酶（GD）、γ-谷氨酰转肽酶（γ-GT）和天冬氨酸转氨酶（AST）浓度的影响。AL14组、AL8组和AL3组的血浆GD活性分别在9周、12周和23周后首次显著升高，γ-GT活性分别在12周、24周和32周后显著升高。AST活性的变化与剂量水平的关系不那么明显。在单次感染的绵羊中，GD和γ-GT都能够检测到10只或更多吸虫移行导致的肝损伤。在慢性亚临床片形吸虫病中，血浆GD和γ-GT活性比AST活性更能敏感地反映肝细胞损伤，并且由于γ-GT具有更高的稳定性，它可能更适合作为一种诊断辅助手段。
