Faivre-Bauman A, Rosenbaum E, Puymirat J, Tixier-Vidal A
C R Seances Acad Sci D. 1980 Mar 31;290(13):885-7.
Fetal neuronal cells dissociated from mouse hypothalamus are able to grow in a serum-free medium for two to four weeks. Several neuronal activities have been measured during the in vitro cell development. Whereas glutamic acid decarboxylase (EC 4.1.1.15) activity disappears, those of thyroliberin (TRH) and tyrosine hydroxylase (EC. 1.14.16.2) remain stable, and acetylcholine transferase one (EC 2.3.1.6) rises earlier than in a serum supplemented medium. These results show that primary cultures grown in serum-free medium offer a promising model for studying neuronal activity differentiation.
从小鼠下丘脑中分离出的胎儿神经细胞能够在无血清培养基中生长两到四周。在体外细胞发育过程中测量了几种神经活动。谷氨酸脱羧酶（EC 4.1.1.15）活性消失，而促甲状腺激素释放激素（TRH）和酪氨酸羟化酶（EC. 1.14.16.2）的活性保持稳定，乙酰胆碱转移酶（EC 2.3.1.6）的活性比在补充血清的培养基中更早升高。这些结果表明，在无血清培养基中生长的原代培养物为研究神经活动分化提供了一个有前景的模型。
