Loudes C, Faivre-Bauman A, Barret A, Grouselle D, Puymirat J, Tixier-Vidal A
Brain Res. 1983 Aug;285(2):231-4. doi: 10.1016/0165-3806(83)90057-3.
Serum-free cultures of mouse hypothalamic cells were used as a model for studying TRH (thyroliberin) secretion in vitro. Supplementation of the culture medium with triiodothyronine, corticosterone and polyunsaturated fatty acids is necessary to obtain a substantial release capacity of TRH neurons. Under these conditions depolarization of the cells with 60 mM K+ results in a calcium-dependent release of immunoreactive TRH.
小鼠下丘脑细胞的无血清培养被用作体外研究促甲状腺激素释放激素（TRH）分泌的模型。向培养基中添加三碘甲状腺原氨酸、皮质酮和多不饱和脂肪酸对于获得TRH神经元的大量释放能力是必要的。在这些条件下，用60 mM K⁺使细胞去极化会导致免疫反应性TRH的钙依赖性释放。
