Levine M M, Rennels M B, Daya V, Hughes T P
J Infect Dis. 1980 Jun;141(6):733-7. doi: 10.1093/infdis/141.6.733.
Controversy exists as to whether all strains of enterotoxigenic Escherichia coli must possess colonization factor antigen pili I or II (CFA/I, CFA/II) in order to be virulent for humans. Failure to detect CFA/I or CFA/II in enterotoxigenic strains from human diarrhea has been explained by some as due to plasmid loss and by others as evidence that CFA/I and CFA/II pili are not prerequisites for human virulence in all enterotoxigenic strains of E. coli. Seven enterotoxigenic and three enteropathogenic strains of E. coli that have been used in volunteer challenge studies were tested for CFA/I, CFA/II, and type 1 somatic pili after culture on solid agar and in broth. Six of the seven enterotoxigenic and two of the three enteropathogenic strains caused diarrhea in humans. Of these eight virulent strains, one produced CFA/I, and one had CFA/II. Threre remained four enterotoxigenic and two enteropathogenic strains that unequivocally caused diarrhea while lacking CFA/I or CFA/II. In such strains other organelles or surface properties must be operative to promote adhesion to and colonization of small intestinal mucosa. CFA/I and CFA/II are not prerequisites of virulence for all E. coli strains that cause diarrhea in humans.
关于是否所有产肠毒素大肠杆菌菌株都必须拥有定居因子抗原菌毛I或II（CFA/I、CFA/II）才能对人类具有致病性，目前存在争议。一些人将未能在人类腹泻的产肠毒素菌株中检测到CFA/I或CFA/II解释为质粒丢失所致，而另一些人则认为这证明CFA/I和CFA/II菌毛并非所有产肠毒素大肠杆菌菌株对人类致病的先决条件。对七株产肠毒素大肠杆菌和三株肠致病性大肠杆菌进行了检测，这些菌株曾用于志愿者激发试验，在固体琼脂和肉汤中培养后，检测其CFA/I、CFA/II和1型菌体菌毛。七株产肠毒素大肠杆菌中的六株和三株肠致病性大肠杆菌中的两株在人类中引起腹泻。在这八株致病菌株中，一株产生CFA/I，一株有CFA/II。仍有四株产肠毒素大肠杆菌和两株肠致病性大肠杆菌在缺乏CFA/I或CFA/II的情况下明确引起腹泻。在这类菌株中，必定有其他细胞器或表面特性起作用，以促进对小肠黏膜的黏附和定植。CFA/I和CFA/II并非所有导致人类腹泻的大肠杆菌菌株致病的先决条件。
