Congote L F, Bruno F, Solomon S
Biol Neonate. 1977;32(5-6):310-8. doi: 10.1159/000241034.
The effects of testosterone and 17beta-estradiol on the synthesis of fetal and adult hemoglobins have been studied using a short-term primary cell culture system of human fetal liver at midgestation. There was a significant 23% increase in incorporation of 59Fe into adult hemoglobin relative to the total after the addition of 5 X 10(-8)M testosterone. 17beta-Estradiol (10(-6)M) lowered the incorporation of 59Fe by 21%. beta-Globin chain synthesis, measured as 3H- or 14C-leucine incorporation into globin chains, was identical in control and testosterone-treated cells and only slightly lower when 17beta-estradiol was added. For this reason the observed changes in adult hemoglobin resulting from the action of testosterone and 17beta-estradiol are caused by an indirect effect either in the final hemoglobin assembly or by small changes in the gamma/alpha-chain ratio which may be undetected by the methodology used.
利用妊娠中期人胎肝短期原代细胞培养系统，研究了睾酮和17β -雌二醇对胎儿及成人血红蛋白合成的影响。加入5×10(-8)M睾酮后，相对于总量，59Fe掺入成人血红蛋白的量显著增加了23%。17β -雌二醇（10(-6)M）使59Fe的掺入量降低了21%。以3H -或14C -亮氨酸掺入珠蛋白链来衡量的β -珠蛋白链合成，在对照细胞和经睾酮处理的细胞中是相同的，而加入17β -雌二醇时仅略有降低。因此，观察到的由睾酮和17β -雌二醇作用导致的成人血红蛋白变化，是由最终血红蛋白组装中的间接作用引起的，或者是由γ/α -链比值的微小变化引起的，而这种变化可能无法通过所使用的方法检测到。
