The effect of temperature during trypsin treatment on viability and multiplication potential of single normal human and chicken fibroblasts.

Both the number of colonies formed from single cells and their average size are affected by the temperature at which nontransformed human and chicken fibroblasts are harvested from monolayer cultures. At constant levels of trypsin activity, treatment of cells at temperatures less than 15 degrees C during all steps from monolayer to subsequent culture markedly improves viability and multiplication potential of single cells.