Griffin B E, Dilworth S M, Ito Y, Novak U
Proc R Soc Lond B Biol Sci. 1980 Nov 19;210(1180):465-76. doi: 10.1098/rspb.1980.0147.
The polyoma virus genome is organized in such a way that the DNA coding capacity of the virus is maximized. Not only are there overlapping genes, but more than one reading frame within a particular region of the genome is used for coding. This is especially apparent in the 'early region', which codes for three of the known viral proteins, the large, middle and small T-antigens. Only a part of this region appears to be necessary for the transformation of cells, and it contains coding information for middle and small T-antigens, but only for a part of large T-antigen. A two-stage model for transformation is proposed which takes into account the major assays for transformation and the viral proteins that are suggested to be involved at each stage. In addition, the virus may induce a host protein to transform by an alternative route. It is suggested that the viral transforming function in polyoma virus could be of host cell origin.
多瘤病毒基因组的组织方式使得病毒的DNA编码能力得以最大化。不仅存在重叠基因，而且基因组特定区域内的多个阅读框都用于编码。这在“早期区域”尤为明显，该区域编码三种已知的病毒蛋白，即大、中、小T抗原。该区域中似乎只有一部分对于细胞转化是必需的，它包含中、小T抗原的编码信息，但仅包含大T抗原的一部分编码信息。提出了一种转化的两阶段模型，该模型考虑了转化的主要检测方法以及在每个阶段被认为涉及的病毒蛋白。此外，病毒可能通过另一种途径诱导宿主蛋白进行转化。有人认为多瘤病毒中的病毒转化功能可能起源于宿主细胞。
