Kobayashi Y, Shigematsu H, Masaki Y, Tateno S, Hiki Y
Virchows Arch B Cell Pathol Incl Mol Pathol. 1980;35(1):45-61. doi: 10.1007/BF02889148.
Experiments were undertaken to clarify whether a large dose of methylprednisolone (MPSL) could have any suppressive effect on progressive Masugi nephritis in the rabbit. Progressive crescentic Masugi nephritis could be induced with high reproducibility by preimmunization with a small amount of nephrotoxic duck gamma-globulin incorporated with complete Freund's adjuvant, followed by an intravenous injection 4 days later. Two groups of rabbits treated with 80 mg/kg of MPSL either before or after the development of proteinuria, showed a significant decrease in both antibody titers and serum creatinine levels during treatment. Histologically, the prominent diffuse intracapillary proliferation and crescent formation observed in controls, were markedly diminished. Accumulations of monocytes in the intra- and extracapillary space were also decreased. These results suggest that suppression of antibody production by a large dose of MPSL is one of its most fundamental actions, and can prevent the processes leading to crescentic glomerular lesions.
开展实验以阐明大剂量甲基强的松龙（MPSL）是否对兔进行性真珠性肾炎有抑制作用。通过用少量与完全弗氏佐剂混合的肾毒性鸭γ球蛋白进行预免疫，4天后静脉注射，可高度重复地诱导出进行性新月体性真珠性肾炎。两组在蛋白尿出现之前或之后接受80mg/kg MPSL治疗的兔子，在治疗期间抗体滴度和血清肌酐水平均显著降低。组织学上，对照组中观察到的显著弥漫性毛细血管内增生和新月体形成明显减少。毛细血管内和毛细血管外间隙中的单核细胞积聚也减少。这些结果表明，大剂量MPSL抑制抗体产生是其最基本的作用之一，并且可以预防导致新月体性肾小球病变的过程。
