Kobayashi Y, Shigematsu H, Masaki Y
Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;43(2):103-19. doi: 10.1007/BF02932948.
Experiments were undertaken to ascertain whether progression of crescentic Masugi nephritis in rabbits could be prevented by the administration of Bredinin (BR). Crescentic glomerulonephritis can be induced with high reproducibility by intramuscular preimmunization on day 1, followed by intravenous injections on days 3 and 5 of nephrotoxic duck gamma-globulin (NTD gamma-gl). 30 rabbits were divided into 3 groups including controls (group 1). Two groups of 10 nephritic rabbits were each treated with 10 mg/kg of BR either after or before the development of proteinuria (groups 2 and 3). In group 3, the onset of proteinuria showed a significant delay and duration of survival was significantly prolonged, compared with controls. Serum antibody titers after day 8 and creatinine levels after day 10, as well as the initial amounts of proteinuria, were also significantly lower during treatment in group 3 than in controls. Histologically, the prominent diffuse intra- and extra-capillary proliferation with monocyte accumulations observed in the control group were markedly diminished in group 3. These results suggest that early treatment in crescentic glomerulonephritis with BR will suppress the production of humoral antibody and prevent progression of the glomerular lesions.
开展实验以确定给予布累迪宁（BR）是否可预防兔新月体性真珠性肾炎的进展。在第1天通过肌肉内预先免疫，随后在第3天和第5天静脉注射肾毒性鸭γ球蛋白（NTDγ-gl），可高度重复性地诱导新月体性肾小球肾炎。30只兔分为3组，包括对照组（第1组）。两组各10只患肾炎的兔，在蛋白尿出现后或出现前分别用10mg/kg的BR进行治疗（第2组和第3组）。与对照组相比，第3组蛋白尿的出现显著延迟，存活时间显著延长。第3组在治疗期间第8天后的血清抗体滴度、第10天后的肌酐水平以及初始蛋白尿的量也显著低于对照组。组织学上，对照组中观察到的显著弥漫性毛细血管内和毛细血管外增殖伴单核细胞积聚在第3组中明显减少。这些结果表明，用BR对新月体性肾小球肾炎进行早期治疗将抑制体液抗体的产生并预防肾小球病变的进展。
