Nambi P, Sharma R K
Endocrinology. 1981 May;108(5):2025-7. doi: 10.1210/endo-108-5-2025.
Low concentrations of ACTH, 7 x 10(-12) M, caused a marked stimulation of the 100,000 x g particulate guanylate cyclase without any detectable change in the adenylate cyclase activity. The lowest concentration of the hormone that elicited adenylate cyclase stimulation was 7 x 10(-10) M, a concentration 100--fold higher than that required to stimulate the guanylate cyclase. Although calcium was found to be obligatory in the hormonally--dependent guanylate cyclase activity, calcium alone could not duplicate the ACTH effect. Sodium nitroprusside and ascorbic acid inhibited the particulate guanylate cyclase activity. While ACTH was unable to stimulate the soluble guanylate cyclase, sodium nitroprusside markedly stimulated this enzyme. From these data, we conclude that the adrenal guanylate cyclase exists in two forms, particulate and soluble. The particulate form is specifically responsive to ACTH, and calcium is one of the essential coupling factors of this hormonally--responsive guanylate cyclase.
低浓度的促肾上腺皮质激素（ACTH，7×10⁻¹² M）可显著刺激100,000×g微粒体鸟苷酸环化酶，而腺苷酸环化酶活性未出现任何可检测到的变化。引发腺苷酸环化酶刺激的激素最低浓度为7×10⁻¹⁰ M，该浓度比刺激鸟苷酸环化酶所需浓度高100倍。尽管发现钙对于激素依赖性鸟苷酸环化酶活性是必需的，但单独的钙无法复制促肾上腺皮质激素的作用。硝普钠和抗坏血酸可抑制微粒体鸟苷酸环化酶活性。虽然促肾上腺皮质激素无法刺激可溶性鸟苷酸环化酶，但硝普钠可显著刺激该酶。根据这些数据，我们得出结论，肾上腺鸟苷酸环化酶以两种形式存在，即微粒体形式和可溶性形式。微粒体形式对促肾上腺皮质激素具有特异性反应，钙是这种激素反应性鸟苷酸环化酶的必需偶联因子之一。
