Snoek G T, van de Poll K W, Voorma H O, van Wijk R
Eur J Biochem. 1981;114(1):27-31. doi: 10.1111/j.1432-1033.1981.tb06166.x.
Synthesis of L-tyrosine:2-oxoglutarate aminotransferase (EC 2.6.1.5) can be induced by N6,O2'-dibutyryl-adenosine 3',5'-monophosphate (Bt2cAMP) in Reuber H35 cell cultures. Actinomycin D fails to block this induction which indicates a target for Bt2cAMP at a posttranscriptional level. We have determined the influence of Bt2cAMP on several translational events during the tyrosine aminotransferase synthesis with the following results. (1) The number of nascent tyrosine aminotransferase chains increased, whereas no effect was measured on the number of nascent total protein chains. (2) The rate of elongation along the tyrosine aminotransferase mRNA and total mRNA is not enhanced by Bt2cAMP. (3) The induced synthesis of tyrosine aminotransferase is more sensitive to the inhibition of elongation. We conclude from our results that Bt2cAMP induces the synthesis of tyrosine aminotransferase by an increase in the rate of initiation on the tyrosine aminotransferase mRNA.
L-酪氨酸的合成：在鲁伯H35细胞培养物中，N6,O2'-二丁酰腺苷3',5'-单磷酸（Bt2cAMP）可诱导2-氧代戊二酸氨基转移酶（EC 2.6.1.5）的产生。放线菌素D无法阻断这种诱导作用，这表明Bt2cAMP在转录后水平有一个作用靶点。我们已经确定了Bt2cAMP在酪氨酸氨基转移酶合成过程中对几个翻译事件的影响，结果如下：（1）新生酪氨酸氨基转移酶链的数量增加，而对新生总蛋白链的数量没有影响。（2）Bt2cAMP不会提高沿酪氨酸氨基转移酶mRNA和总mRNA的延伸速率。（3）诱导合成的酪氨酸氨基转移酶对延伸抑制更敏感。我们从结果中得出结论，Bt2cAMP通过提高酪氨酸氨基转移酶mRNA的起始速率来诱导酪氨酸氨基转移酶的合成。
