Diebler M F, Morot-Gaudry Y
Brain Res. 1981 Mar 30;209(2):482-4. doi: 10.1016/0006-8993(81)90174-8.
Using thermal inactivation, further characterization studies on acetyl-CoA synthetase found in isolated nerve endings and in mitochondria obtained from the electric organ of Torpedo marmorata were made. They confirm different properties of the two enzymatic forms. Moreover, the acetyl-CoA synthetase activity found in the cytosol of disrupted synaptosomes exhibits a homogeneous thermic inactivation curve which corresponds to a single enzymatic form. It has the same localization as choline acetyltransferase.
利用热失活技术，对从电鳐电器官分离出的神经末梢和线粒体中发现的乙酰辅酶A合成酶进行了进一步的特性研究。这些研究证实了两种酶形式具有不同的特性。此外，在破碎的突触体胞质溶胶中发现的乙酰辅酶A合成酶活性呈现出一条均匀的热失活曲线，这对应于单一的酶形式。它与胆碱乙酰转移酶具有相同的定位。
