人类染色体交换畸变的可视化技术。

Technique for the visualization of exchange aberrations in human chromosomes.

作者信息

Fischer P, Kim M A

出版信息

Exp Pathol (Jena). 1975;10(3-4):216-9. doi: 10.1016/s0014-4908(75)80025-1.

Abstract

A technique for the visualization of chromatid exchange in human chromosomes is described. Additon of the base analogue 5-bromodeoxyuridine (BUdR) into PHA stimulated lymphocyte cultures and subsequent staining with the benzimidazol compound "33258 Hoechst", and Giemsa, results in differential fluorescence or staining of sister chromatids in second division metaphases. This technique allows an accurate scoring of sister chromatid exchanges, and could be used in the evaluation of the effect of external agents on the induction of previously unrecognized chromatid exchanges in human chromosomes.

摘要

本文描述了一种用于观察人类染色体中染色单体交换的技术。将碱基类似物5-溴脱氧尿苷（BUdR）添加到PHA刺激的淋巴细胞培养物中，随后用苯并咪唑化合物“33258 Hoechst”和吉姆萨染色，可使第二次分裂中期的姐妹染色单体产生差异荧光或染色。该技术能够准确计分姐妹染色单体交换情况，可用于评估外部因素对诱导人类染色体中先前未识别的染色单体交换的影响。