Daniel J Y, Vignon F, Assenmacher I, Rochefort H
Steroids. 1977 Nov;30(5):703-9. doi: 10.1016/0039-128x(77)90059-9.
Androgen receptors have been found in duck preen glands by using dextran-coated charcoal adsorption. They bound DHT with high affinity (KD = 0.2 nM), limited capacity (45 fmoles/mgP) and good specificity. They sedimented at 8 S in a sucrose gradient and were destroyed by pronase digestion and heating. An estrogen receptor having different binding specificity was also demonstrated. On the basis of a marked annual cycle of gonadal activity in ducks, this system appears appropriate for studying the regulation of sex steroid hormone receptors.
通过使用葡聚糖包被活性炭吸附法，已在鸭尾脂腺中发现雄激素受体。它们以高亲和力（KD = 0.2 nM）、有限容量（45飞摩尔/毫克蛋白）和良好的特异性结合双氢睾酮。它们在蔗糖梯度中以8 S沉降，并被链霉蛋白酶消化和加热破坏。还证实了一种具有不同结合特异性的雌激素受体。基于鸭性腺活动明显的年度周期，该系统似乎适合用于研究性甾体激素受体的调节。
