Kishida S, Fukushima D K
Steroids. 1977 Dec;30(6):741-9. doi: 10.1016/s0039-128x(77)80020-2.
A sensitive and reliable radioimmunoassay for urine and plasma 6beta-hydroxycortisol has been developed. Antiserum showing high specificity against 6beta-hydroxycortisol was produced in rabbits immunized with 6beta-hydroxycortisol 21-hemisuccinate-bovine serum albumin. The sensitivity of the assay was 25 pg on a diluted sample equivalent to 1 microliter of urine, and on 50 microliter of plasma after separation by celite chromatography. The intra- and inter-assay coefficients of variation for urine were 4.8 and 6.7% and those for plasma were 4.2 and 12.1%. Concentrations were determined in patients with bronchogenic carcinoma, in patients treated with dilantin, in neonates, and in infants aged 5-12 months.
已开发出一种灵敏且可靠的尿液和血浆6β-羟基皮质醇放射免疫测定法。用6β-羟基皮质醇21-半琥珀酸-牛血清白蛋白免疫兔子，产生了对6β-羟基皮质醇具有高特异性的抗血清。该测定法对稀释后相当于1微升尿液的样品的灵敏度为25皮克，对经硅藻土色谱分离后的50微升血浆的灵敏度为25皮克。尿液测定的批内和批间变异系数分别为4.8%和6.7%，血浆测定的批内和批间变异系数分别为4.2%和12.1%。对支气管癌患者、接受苯妥英治疗的患者、新生儿以及5至12个月大的婴儿进行了浓度测定。
