Al-Dahash S Y, David J S
Vet Rec. 1977 Oct 29;101(18):361-3. doi: 10.1136/vr.101.18.361.
Histochemical methods have been used to measure delta5-3beta-hydroxy-steroid dehydrogenase (3beta-HSD) activity in order to establish sites and degree of steroid hormone synthesis in cystic ovaries, corpora lutea and uteri. When granulosa cell layers were present they showed enzymic activity indicating their ability to produce steroids. When both were absent no activity could be seen. Corpora lutea either from ovaries associated with an ovarian cyst or from normally cyclic cows showed varying degrees of activity. When the corpuluteum was immature, activity was low, when mature it was very high and when regressing the activity was declining. No activity was seen in uteri.
已采用组织化学方法来测量δ5-3β-羟基类固醇脱氢酶（3β-HSD）的活性，以便确定囊性卵巢、黄体和子宫中类固醇激素合成的部位和程度。当存在颗粒细胞层时，它们表现出酶活性，表明其具有产生类固醇的能力。当两者都不存在时，则看不到活性。来自与卵巢囊肿相关的卵巢或正常周期性奶牛的黄体表现出不同程度的活性。当黄体不成熟时，活性较低；当成熟时，活性非常高；当退化时，活性则在下降。在子宫中未观察到活性。
