Kleinerman J, Marchevsky A M, Thornton J
Am Rev Respir Dis. 1981 Oct;124(4):458-62. doi: 10.1164/arrd.1981.124.4.458.
A method of quantitating the airways amine precursor uptake decarboxylase (APUD) cells is proposed that relates these cells to total airway epithelial cells or airway length. In control rats, these values were 0.13 APUD cells/100 epithelial cells in the trachea, 0.03 in the large airways, and 0.02 in the small airways. In order to evaluate the ability of this method to determine changes in number of APUD cells, rats were exposed to diethylnitrosamine (DEN) and nitrogen dioxide (NO2), which have been reported to increase the number of airways APUD cells. The NO2 exposure was associated with a twofold increase in APUD cells of the trachea only without associated epithelial hyperplasia; DEN produced marked epithelial hyperplasia in the trachea and bronchi with a disproportionate increase in APUD cells in the bronchial epithelium only. The basis of this differential effect requires further study.
提出了一种定量气道胺前体摄取脱羧酶（APUD）细胞的方法，该方法将这些细胞与气道上皮细胞总数或气道长度相关联。在对照大鼠中，这些数值在气管中为0.13个APUD细胞/100个上皮细胞，在大气道中为0.03，在小气道中为0.02。为了评估该方法确定APUD细胞数量变化的能力，将大鼠暴露于二乙基亚硝胺（DEN）和二氧化氮（NO2）中，据报道这两种物质会增加气道APUD细胞的数量。仅暴露于NO2会使气管中的APUD细胞数量增加两倍，且无相关的上皮增生；DEN使气管和支气管出现明显的上皮增生，仅支气管上皮中的APUD细胞数量不成比例地增加。这种差异效应的基础需要进一步研究。
