Clayton C J, McNeill T H, Sladek J R
Cell Tissue Res. 1981;220(2):223-30. doi: 10.1007/BF00210504.
Immunocytochemical staining of luteinizing hormone-releasing hormone (LHRH), somatostatin, and neurophysin was compared in rat brains fixed with 1) formalin, 2) Bouin's solution, 3) freeze-dried (FD), or 4) freeze-dried + paraformaldehyde vapor perfused (FDV). The distribution of LHRH fibers was similar in all preparations; however, beads of granular reaction product often appeared finer and more numerous in the median eminence of FD- and FDV brains. Positively stained LHRH perikarya were not observed in any of the preparations. In contrast, somatostatin-immunoreactive perikarya were present in the fluid-fixed and FD brains, although few were observed in FDV brains. Somatostatin-immunoreactive fibers were present in all preparations, but appeared most numerous in the median eminence of FD brains. Staining of neurophysin-containing perikarya and fibers was similar in all preparations. These observations suggest that the FD brain can provide a suitable tissue substrate for immunocytochemistry, demonstrating staining comparable to or surpassing that of more conventional preparations. However, staining of antigens in FD brain was not uniformly successful and may depend on stereochemical characteristics of each antigen as well as properties of the primary antisera used in the staining procedure.
对用以下方法固定的大鼠脑进行黄体生成素释放激素（LHRH）、生长抑素和神经垂体素的免疫细胞化学染色比较：1）福尔马林，2）波因氏液，3）冷冻干燥（FD），或4）冷冻干燥+多聚甲醛蒸汽灌注（FDV）。在所有制剂中，LHRH纤维的分布相似；然而，在FD脑和FDV脑的正中隆起处，颗粒状反应产物的珠状物通常显得更细且更多。在任何制剂中均未观察到阳性染色的LHRH核周体。相比之下，生长抑素免疫反应性核周体存在于液体固定和FD脑中，尽管在FDV脑中观察到的很少。生长抑素免疫反应性纤维存在于所有制剂中，但在FD脑的正中隆起处似乎最多。含神经垂体素的核周体和纤维的染色在所有制剂中相似。这些观察结果表明，FD脑可为免疫细胞化学提供合适的组织底物，显示出与更传统制剂相当或更好的染色效果。然而，FD脑中抗原的染色并非始终成功，可能取决于每种抗原的立体化学特征以及染色过程中使用的一抗的特性。
