McNeill T, Kozlowski G P, Abel J H, Zimmerman E A
Endocrinology. 1976 Nov;99(5):1323-32. doi: 10.1210/endo-99-5-1323.
Localization studies of the hypothalamohypophysial and tuberoinfundibular neurosecretory systems were performed in the adult male mallard duck with an immunoperoxidase techinque for the demonstration of neurophysin (NP) and gonado-tropin-releasing hormone (Gn-RH) and with aldehyde fuchsin for the staining of neuosecretory material (NSM). A comparison was made between the distribution of NSM stained with aldehyde fuchsin and NP seen by immunocytochemistry. The magnocellular perikarya of the supraoptic (SON) and paraventricular (PVN) nuclei, the zona externa of the anterior median eminence (ME), the fiber layer of both the anterior and posterior ME, and small neurons in the tractus quintofrontalis were stained by both the immunoperoxidase method for NP and by the aldehyde fuchsin stain. In contrast, the parvocellular neurons of the PVN, extra-hypothalamic neurosecretory fibers dorsal to the anterior commissure in the septal region and tanycytes lining the ventral 1/3 of the third ventricle at the level of the anterior ME, were stained only by the immunocytochemical procedure for NP. These observations indicate that immunocytochemistry is more sensitive than aldehyde fuchsin staining for detecting low concentrations of NP in cells and tissues, but the two techniques produce comparable results where the concentration of the NP is relatively high. Two populations of beaded axons containing Gn-RH were distributed throughout the zone externa of both the anterior and posterior ME. One group of fibers paralleled the hypothalamo-hypophysial neurosecretory tract whereas the other was distributed in the contact zone of the ME. Immunoreactive Gn-RH was found in the cytoplasm of a sparse population of cell bodies in the dorsolateral portion of the arcuate nucleus as well as in the axons that project from this nucleus ventrally towards the ME.
利用免疫过氧化物酶技术检测神经垂体素（NP）和促性腺激素释放激素（Gn-RH），并使用醛复红对神经分泌物质（NSM）进行染色，对成年雄性绿头鸭的下丘脑 - 垂体和结节 - 漏斗神经分泌系统进行了定位研究。对用醛复红染色的NSM分布与免疫细胞化学检测到的NP分布进行了比较。视上核（SON）和室旁核（PVN）的大细胞性胞体、正中隆起前部（ME）的外层、ME前后部的纤维层以及额五束中的小神经元，通过NP免疫过氧化物酶法和醛复红染色均被染色。相比之下，PVN的小细胞性神经元、隔区前连合背侧的下丘脑外神经分泌纤维以及ME前部水平第三脑室腹侧1/3内衬的室管膜细胞，仅通过NP免疫细胞化学方法被染色。这些观察结果表明，免疫细胞化学在检测细胞和组织中低浓度NP方面比醛复红染色更敏感，但在NP浓度相对较高时，这两种技术产生的结果相当。两组含有Gn-RH的串珠状轴突分布在ME前后部的整个外层。一组纤维与下丘脑 - 垂体神经分泌束平行，而另一组分布在ME的接触区。在弓状核背外侧部分稀疏的细胞体细胞质中以及从该核腹侧投射到ME的轴突中发现了免疫反应性Gn-RH。
