血管加压素对肝脏脂肪生成和乙酰辅酶A羧化酶的刺激作用。
Stimulation of hepatic lipogenesis and acetyl-coenzyme A carboxylase by vasopressin.
作者信息
Assimacopoulos-Jeannet F, Denton R M, Jeanrenaud B
出版信息
Biochem J. 1981 Sep 15;198(3):485-90. doi: 10.1042/bj1980485.
Abstract
The effect of vasopressin on the short-term regulation of fatty acid synthesis was studied in isolated hepatocytes from rats fed ad libitum. Vasopressin stimulates fatty acid synthesis by 30-110%. This increase is comparable with that obtained with insulin. Angiotensin also stimulates fatty acid synthesis, whereas phenylephrine does not. The dose-response curve for vasopressin-stimulated lipogenesis is similar to the dose-response curve for glycogenolysis and release of lactate plus pyruvate. Vasopression also stimulates acetyl-CoA carboxylase activity in a dose-dependent manner. Vasopressin does not relieve glucagon-inhibited lipogenesis, whereas insulin does. The action of vasopressin on hepatic lipogenesis is decreased, but not suppressed, in Ca2+-depleted hepatocytes. The results suggest that vasopressin acts on lipogenesis by increasing availability of lipogenic substrate (lactate + pyruvate) and by activating acetyl-CoA carboxylase.
摘要
在随意进食的大鼠分离肝细胞中研究了血管加压素对脂肪酸合成短期调节的作用。血管加压素可刺激脂肪酸合成增加30%-110%。这种增加与胰岛素所引起的增加相当。血管紧张素也刺激脂肪酸合成，而苯肾上腺素则无此作用。血管加压素刺激脂肪生成的剂量-反应曲线与糖原分解及乳酸加丙酮酸释放的剂量-反应曲线相似。血管加压素还以剂量依赖方式刺激乙酰辅酶A羧化酶活性。血管加压素不能解除胰高血糖素对脂肪生成的抑制作用，而胰岛素则可以。在钙离子缺乏的肝细胞中，血管加压素对肝脏脂肪生成的作用减弱，但未被抑制。结果表明，血管加压素通过增加脂肪生成底物（乳酸+丙酮酸）的可用性以及激活乙酰辅酶A羧化酶来作用于脂肪生成。
相似文献
1
Stimulation of hepatic lipogenesis and acetyl-coenzyme A carboxylase by vasopressin.血管加压素对肝脏脂肪生成和乙酰辅酶A羧化酶的刺激作用。
Biochem J. 1981 Sep 15;198(3):485-90. doi: 10.1042/bj1980485.
2
Studies on the assay, activity and sedimentation behaviour of acetyl-CoA carboxylase from isolated hepatocytes incubated with insulin or glucagon.关于用胰岛素或胰高血糖素孵育的分离肝细胞中乙酰辅酶A羧化酶的测定、活性及沉降行为的研究。
Biochem J. 1984 Aug 1;221(3):869-74. doi: 10.1042/bj2210869.
3
Both insulin and epidermal growth factor stimulate lipogenesis and acetyl-CoA carboxylase activity in isolated adipocytes. Importance of homogenization procedure in avoiding artefacts in acetyl-CoA carboxylase assay.胰岛素和表皮生长因子均可刺激分离的脂肪细胞中的脂肪生成及乙酰辅酶A羧化酶活性。乙酰辅酶A羧化酶测定中匀浆程序对于避免人为误差的重要性。
Biochem J. 1986 Mar 1;234(2):279-84. doi: 10.1042/bj2340279.
4
Inhibition of hepatic lipogenesis by salicylate.水杨酸盐对肝脏脂肪生成的抑制作用。
Toxicology. 1982;24(1):33-43. doi: 10.1016/0300-483x(82)90060-9.
5
Short-term effects of insulin and glucagon on lipid synthesis in isolated rat hepatocytes. Covariance of acetyl-CoA carboxylase activity and the rate of 3H2O incorporation into fatty acids.胰岛素和胰高血糖素对分离的大鼠肝细胞脂质合成的短期影响。乙酰辅酶A羧化酶活性与3H2O掺入脂肪酸速率的协方差。
FEBS Lett. 1978 Nov 15;95(2):326-30. doi: 10.1016/0014-5793(78)81022-9.
6
Insulin activation of lipogenesis in isolated mammary acini from lactating rats fed on a high-fat diet. Evidence that acetyl-CoA carboxylase is a site of action.胰岛素对高脂饮食喂养的泌乳大鼠分离乳腺腺泡中脂肪生成的激活作用。乙酰辅酶A羧化酶是作用位点的证据。
Biochem J. 1987 Mar 15;242(3):905-11. doi: 10.1042/bj2420905.
7
Fatty acid inhibition of hormonal induction of acetyl-coenzyme A carboxylase in hepatocyte monolayers.脂肪酸对肝细胞单层中激素诱导的乙酰辅酶A羧化酶的抑制作用。
Arch Biochem Biophys. 1986 Apr;246(1):82-9. doi: 10.1016/0003-9861(86)90451-0.
8
Acute alloxan diabetes alters the activity but not the total quantity of acetyl CoA carboxylase in rat liver.急性四氧嘧啶糖尿病会改变大鼠肝脏中乙酰辅酶A羧化酶的活性，但不会改变其总量。
J Nutr. 1987 Nov;117(11):1976-81. doi: 10.1093/jn/117.11.1976.
9
Time course of hormonal effects on acetyl-CoA carboxylase as measured in digitonin-permeabilized rat hepatocytes.在洋地黄皂苷通透化的大鼠肝细胞中测量激素对乙酰辅酶A羧化酶作用的时间进程。
Horm Metab Res. 1989 Nov;21(11):602-5. doi: 10.1055/s-2007-1009298.
10
Role of protein synthesis in the carbohydrate-induced changes in the activities of acetyl-CoA carboxylase and hydroxymethylglutaryl-CoA reductase in cultured rat hepatocytes.蛋白质合成在碳水化合物诱导的培养大鼠肝细胞中乙酰辅酶A羧化酶和羟甲基戊二酸单酰辅酶A还原酶活性变化中的作用。
Biochem J. 1985 May 1;227(3):939-47. doi: 10.1042/bj2270939.
引用本文的文献
1
Molecular Interaction Between Vasopressin and Insulin in Regulation of Metabolism: Impact on Cardiovascular and Metabolic Diseases.血管加压素与胰岛素在代谢调节中的分子相互作用：对心血管和代谢疾病的影响
Int J Mol Sci. 2024 Dec 11;25(24):13307. doi: 10.3390/ijms252413307.
2
Vasopressin and hydration play a major role in the development of glucose intolerance and hepatic steatosis in obese rats.加压素和水合作用在肥胖大鼠葡萄糖不耐受和肝脂肪变性的发展中起主要作用。
Diabetologia. 2015 May;58(5):1081-90. doi: 10.1007/s00125-015-3496-9. Epub 2015 Jan 27.
3
Effects of inhibition of protein synthesis by cycloheximide on lipogenesis in mammary gland and liver of lactating rats.放线菌酮抑制蛋白质合成对泌乳大鼠乳腺和肝脏脂肪生成的影响。
Biochem J. 1982 May 15;204(2):417-23. doi: 10.1042/bj2040417.
4
Effects of adrenergic agents, vasopressin and ionophore A23187, on the phosphorylation of, and flux through, phenylalanine hydroxylase in rat liver cells.肾上腺素能药物、血管加压素和离子载体A23187对大鼠肝细胞中苯丙氨酸羟化酶磷酸化及通量的影响。
Biochem J. 1984 Apr 1;219(1):87-90. doi: 10.1042/bj2190087.
5
Studies on the assay, activity and sedimentation behaviour of acetyl-CoA carboxylase from isolated hepatocytes incubated with insulin or glucagon.关于用胰岛素或胰高血糖素孵育的分离肝细胞中乙酰辅酶A羧化酶的测定、活性及沉降行为的研究。
Biochem J. 1984 Aug 1;221(3):869-74. doi: 10.1042/bj2210869.
6
Induction of fatty acid synthetase and acetyl-CoA carboxylase by isolated rat liver cells.分离的大鼠肝细胞对脂肪酸合成酶和乙酰辅酶A羧化酶的诱导作用。
Mol Cell Biochem. 1983;53-54(1-2):307-25. doi: 10.1007/BF00225262.
7
Changes in the proportion of acetyl-CoA carboxylase in the active form in rat liver. Effect of starvation, lactation and weaning.大鼠肝脏中活性形式的乙酰辅酶A羧化酶比例的变化。饥饿、哺乳和断奶的影响。
Biochem J. 1982 Jun 15;204(3):757-64. doi: 10.1042/bj2040757.
8
Regulation of acetyl-CoA carboxylase in rat mammary gland. Effects of starvation and of insulin and prolactin deficiency on the fraction of the enzyme in the active form in vivo.大鼠乳腺中乙酰辅酶A羧化酶的调节。饥饿以及胰岛素和催乳素缺乏对体内活性形式酶的比例的影响。
Biochem J. 1982 Apr 15;204(1):273-80. doi: 10.1042/bj2040273.
9
Intramitochondrial regulation of fatty acid beta-oxidation occurs between flavoprotein and ubiquinone. A role for changes in the matrix volume.脂肪酸β-氧化的线粒体内调节发生在黄素蛋白和泛醌之间。基质体积变化的作用。
Biochem J. 1986 Nov 1;239(3):559-65. doi: 10.1042/bj2390559.
10
Stimulation of fatty acid synthesis by 4 beta-phorbol-12-myristate-13-acetate in isolated rat hepatocytes.4-β-佛波醇-12-肉豆蔻酸酯-13-乙酸酯对分离的大鼠肝细胞中脂肪酸合成的刺激作用。
Lipids. 1986 May;21(5):366-7. doi: 10.1007/BF02535703.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
3
Interactions between vasopressin and glucagon on ketogenesis and oleate metabolism in isolated hepatocytes from fed rats.血管升压素与胰高血糖素对喂食大鼠分离肝细胞中酮体生成和油酸代谢的相互作用。
Biochem J. 1980 Feb 15;186(2):621-4. doi: 10.1042/bj1860621.
4
Evidence for phosphorylation and activation of acetyl CoA carboxylase by a membrane-associated cyclic AMP-independent protein kinase. Relationship to the activation of acetyl CoA carboxylase by insulin.膜相关的非环磷酸腺苷依赖性蛋白激酶对乙酰辅酶A羧化酶进行磷酸化和激活的证据。与胰岛素对乙酰辅酶A羧化酶激活的关系。
FEBS Lett. 1981 Feb 23;124(2):145-50. doi: 10.1016/0014-5793(81)80123-8.
5
Liver acetyl CoA carboxylase and fatty acid synthetase: relative activities in the normal state and in hereditary obesity.肝脏乙酰辅酶A羧化酶和脂肪酸合成酶：正常状态及遗传性肥胖中的相对活性
Biochem Biophys Res Commun. 1967 Sep 7;28(5):682-6. doi: 10.1016/0006-291x(67)90369-5.
6
Removal of fatty acids from serum albumin by charcoal treatment.通过活性炭处理从血清白蛋白中去除脂肪酸。
J Biol Chem. 1967 Jan 25;242(2):173-81.
7
Perfusion in situ with tritium oxide to measure hepatic lipogenesis and lipid secretion. Normal and orotic acid-fed rats.用氧化氚原位灌注以测量肝脏脂肪生成和脂质分泌。正常大鼠和喂食乳清酸的大鼠。
J Biol Chem. 1966 Jun 25;241(12):2891-9.
8
Citrate and the conversion of carbohydrate into fat.柠檬酸盐与碳水化合物向脂肪的转化
Biochem Soc Symp. 1968;27:61-86.
9
Fatty acid synthesis in adipose tissue incubated in tritiated water.在氚化水中孵育的脂肪组织中的脂肪酸合成。
Biochemistry. 1968 Oct;7(10):3708-17. doi: 10.1021/bi00850a050.
Zotero 插件