谷氨酰胺合成酶基因L及其他调控位点对产气克雷伯菌中谷氨酰胺合成酶基因A-乳糖操纵子融合转录调控的影响。
Effects of glnL and other regulatory loci on regulation of transcription of glnA-lacZ fusions in Klebsiella aerogenes.
作者信息
Goldie H, Magasanik B
出版信息
J Bacteriol. 1982 Apr;150(1):231-8. doi: 10.1128/jb.150.1.231-238.1982.
Abstract
Mutants of Klebsiella aerogenes containing genetic fusions of glnA to lacZ were isolated by using Mu dl (lac, bla) bacteriophage and a Mu Kmr helper phage with the host range of bacteriophage P1. Synthesis of beta-galactosidase in these strains is regulated in response to nitrogen metabolites and regulatory gln loci and is rendered constitutive by a mutation in the linked glnL gene. Complementation studies indicated that glnL is a separate locus from glnA and glnG and that insertions in glnA are partially polar on glnL expression. These results support the hypothesis that glnA, glnL, and glnG are organized in an operon with multiple promoters.
摘要
利用Mu dl(lac, bla)噬菌体和具有噬菌体P1宿主范围的Mu Kmr辅助噬菌体，分离出了产气克雷伯菌中含有谷氨酰胺合成酶基因（glnA）与β-半乳糖苷酶基因（lacZ）基因融合的突变体。这些菌株中β-半乳糖苷酶的合成受氮代谢物和调节性谷氨酰胺基因座的调控，并且由于连锁的谷氨酰胺调节基因（glnL）发生突变而变为组成型表达。互补研究表明，glnL是一个与glnA和glnG不同的基因座，并且glnA中的插入对glnL的表达具有部分极性效应。这些结果支持了glnA、glnL和glnG以具有多个启动子的操纵子形式组织的假说。
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