大肠杆菌磷酸烯醇式丙酮酸羧激酶基因座转录的调控：pck-lacZ操纵子融合的研究
Regulation of transcription of the Escherichia coli phosphoenolpyruvate carboxykinase locus: studies with pck-lacZ operon fusions.
作者信息
Goldie H
出版信息
J Bacteriol. 1984 Sep;159(3):832-6. doi: 10.1128/jb.159.3.832-836.1984.
Abstract
Mutants of Escherichia coli containing genetic fusions of lacZ to the pck (phosphoenolpyruvate carboxykinase) locus were isolated by using Mu d(lacZ Ampr) bacteriophage. Synthesis of beta-galactosidase in these strains is regulated by cyclic AMP and glucose (catabolite repression). Synthesis of beta-galactosidase by pck-lacZ fusions was induced in log-phase cells growing on gluconeogenic media, was repressed by glucose, and was also induced up to 100-fold at the onset of stationary phase in LB medium. This stationary-phase induction required cyclic AMP and some other unknown regulatory signal.
摘要
通过使用 Mud(lacZ Ampr)噬菌体分离出了含有 lacZ 与 pck（磷酸烯醇丙酮酸羧激酶）基因座基因融合的大肠杆菌突变体。这些菌株中β-半乳糖苷酶的合成受环腺苷酸和葡萄糖（分解代谢物阻遏）调控。pck-lacZ 融合体在糖异生培养基上生长的对数期细胞中诱导β-半乳糖苷酶的合成，被葡萄糖抑制，并且在 LB 培养基中稳定期开始时也被诱导高达 100 倍。这种稳定期诱导需要环腺苷酸和一些其他未知的调节信号。
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