Scollay R
J Immunol. 1982 Apr;128(4):1566-70.
To gain information on the lineage relationship of cells leaving the thymus, we studied the phenotype of thymus emigrants within hours of their exit. The migrants were identified in the peripheral lymphoid organs by their fluorescence, 3 to 4 hr after intrathymic injection of a solution of fluorescein isothiocyanate, a technique that initially only labels thymocytes. Migrants identified in this way were analyzed with rhodamine-anti-Thy-1 or rhodamine peanut agglutinin (PNA). They were found to express Thy-1 antigen and PNA binding sites at levels very similar to those found on the majority of peripheral T cells or medullary thymocytes and quite different from cortical thymocytes. Taken together with our previous experiments on Lyt-1, Lyt-2, and H-2 levels, the data show that cells leaving the thymus are quite mature in phenotype and are indistinguishable from peripheral T cells by all the criteria examined.
为了获取有关离开胸腺的细胞谱系关系的信息，我们在胸腺迁出细胞离开胸腺后的数小时内研究了它们的表型。在胸腺内注射异硫氰酸荧光素溶液3至4小时后，通过荧光在外周淋巴器官中鉴定出迁移细胞，该技术最初仅标记胸腺细胞。用罗丹明抗Thy-1或罗丹明花生凝集素（PNA）分析以这种方式鉴定出的迁移细胞。发现它们表达Thy-1抗原和PNA结合位点的水平与大多数外周T细胞或髓质胸腺细胞上发现的水平非常相似，与皮质胸腺细胞有很大不同。结合我们之前关于Lyt-1、Lyt-2和H-2水平的实验，数据表明离开胸腺的细胞在表型上相当成熟，并且根据所有检测标准与外周T细胞无法区分。
