Haars R, Kronenberg M, Gallatin W M, Weissman I L, Owen F L, Hood L
J Exp Med. 1986 Jul 1;164(1):1-24. doi: 10.1084/jem.164.1.1.
Rearrangement and expression of the T cell antigen receptor and the gamma genes during T cell ontogeny is a regulated process; the gamma genes are rearranged and expressed first, followed by the beta and then the alpha genes. Expression of both functional alpha and beta gene RNA first occurs at day 17 of gestation, along with the expression of T3 delta chain RNA. T cell antigen receptor gene rearrangements occur primarily or exclusively in the thymus, although some gamma gene rearrangements occur outside the thymus in fetal liver cells that may be committed T cell progenitors. There is no gross difference in the extent of beta and gamma gene rearrangements in the adult thymocyte subpopulations that were analyzed, despite the fact that some of these populations cannot respond to antigen and never emigrate from the thymus. Quantitative analysis of rearrangements in total adult thymocyte DNA shows that beta gene rearrangements generally occur on both chromosomal homologs, and that rearrangements occur preferentially to the J beta 2 gene segment cluster.
在T细胞个体发育过程中，T细胞抗原受体和γ基因的重排与表达是一个受调控的过程；γ基因首先进行重排和表达，随后是β基因，然后是α基因。功能性α和β基因RNA的表达最初出现在妊娠第17天，同时伴有T3δ链RNA的表达。T细胞抗原受体基因重排主要或仅发生在胸腺中，尽管一些γ基因重排在胸腺外的胎儿肝细胞中发生，这些细胞可能是定向的T细胞祖细胞。在所分析的成年胸腺细胞亚群中，β和γ基因重排的程度没有明显差异，尽管其中一些亚群不能对抗原作出反应且从未离开胸腺。对成年胸腺细胞总DNA重排的定量分析表明，β基因重排通常发生在两条染色体同源物上，并且重排优先发生于Jβ2基因片段簇。
