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淡水蛤Anodonta cataracta外套膜中HCO3(-)-ATP酶的亚细胞定位与特性分析

Subcellular localization and characterization of HCO3(-)-ATPase from the mantle of the freshwater clam, Anodonta cataracta.

作者信息

Wheeler A P, Harrison E W

出版信息

Comp Biochem Physiol B. 1982;71(4):629-36. doi: 10.1016/0305-0491(82)90473-4.

DOI:10.1016/0305-0491(82)90473-4
PMID:6123405
Abstract
  1. HCO3(-)-stimulated ATPase activity was demonstrated in mantle tissue of the freshwater clam, Anodonta cataracta. 2. Calcium (1 mM) slightly inhibited and SCN- completely inhibited HCO3(-)-stimulation of the enzyme. 3. ATPase activity had a Km of 6.8 mM for HCO3(-)-activation and was inhibited at HCO3(-)-concentrations greater than 20 mM. 4. Subcellular fractionation studies revealed the presence of both a mitochondrial and a non-mitochondrial HCO3(-)-ATPase.
摘要
  1. 在淡水蛤类白内障无齿蚌的外套膜组织中证实了HCO3(-)刺激的ATP酶活性。2. 钙(1 mM)对该酶的HCO3(-)刺激有轻微抑制作用,而SCN-则完全抑制。3. ATP酶活性对HCO3(-)激活的Km为6.8 mM,在HCO3(-)浓度大于20 mM时受到抑制。4. 亚细胞分级分离研究表明存在线粒体和非线粒体的HCO3(-)-ATP酶。

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引用本文的文献

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Presence of an extramitochondrial anion-stimulated ATPase in the rabbit kidney: localization along the nephron and effect of corticosteroids.兔肾中存在一种线粒体外阴离子刺激的ATP酶:沿肾单位的定位及皮质类固醇的作用。
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