Gardiol A, Arias A, Cerveñansky C, Martínez-Drets G
J Bacteriol. 1982 Sep;151(3):1621-3. doi: 10.1128/jb.151.3.1621-1623.1982.
A succinate dehydrogenase mutant strain of Rhizobium meliloti was isolated after nitrosoguanidine mutagenesis. It failed to grow on succinate, glutamate, acetate, pyruvate, or arabinose but grew on glucose, sucrose, fructose, and other carbohydrates. The mutant strain showed delayed nodulation of lucerne plants, and the nodules were white and ineffective. A spontaneous revertant strain of normal growth phenotype induced red and effective nodules.
经亚硝基胍诱变后,分离得到了苜蓿根瘤菌的琥珀酸脱氢酶突变株。该突变株不能在琥珀酸、谷氨酸、乙酸、丙酮酸或阿拉伯糖上生长,但能在葡萄糖、蔗糖、果糖和其他碳水化合物上生长。突变株使苜蓿植株的结瘤延迟,且根瘤呈白色,固氮无效。一个具有正常生长表型的自发回复突变株能诱导出红色且有效的根瘤。