苜蓿根瘤菌α-酮戊二酸脱氢酶突变体
alpha-Ketoglutarate dehydrogenase mutant of Rhizobium meliloti.
作者信息
Duncan M J, Fraenkel D G
出版信息
J Bacteriol. 1979 Jan;137(1):415-9. doi: 10.1128/jb.137.1.415-419.1979.
Abstract
A mutant of Rhizobium meliloti selected as unable to grow on L-arabinose also failed to grow on acetate or pyruvate. It grew, but slower than the parental strain, on many other carbon sources. Assay showed it to lack alpha-ketoglutarate dehydrogenase (kgd) activity, and revertants of normal growth phenotype contained the activity again. Other enzymes of the tricarboxylic acid cycle and of the glyoxylate cycle were present in both mutant and parent strains. Enzymes of pyruvate metabolism were also assayed. L-Arabinose degradation in R. meliloti was found to differ from the known pathway in R. japonicum, since the former strain lacked 2-keto-o-deoxy-L-arabonate aldolase but contained alpha-ketoglutarate semialdehyde dehydrogenase; thus, it is likely that R. meliloti has the L-arabinose pathway leading to alpha-ketoglutarate rather than the one to glycolaldehyde and pyruvate. This finding accounts for the L-arabinose negativity of the mutant. Resting cells of the mutant were able to metabolize the three substrates which did not allow growth.
摘要
一种被选作不能在L-阿拉伯糖上生长的苜蓿根瘤菌突变体，在乙酸盐或丙酮酸盐上也不能生长。它能在许多其他碳源上生长，但比亲本菌株生长得慢。检测表明它缺乏α-酮戊二酸脱氢酶（kgd）活性，具有正常生长表型的回复突变体又具有该活性。三羧酸循环和乙醛酸循环的其他酶在突变体菌株和亲本菌株中都存在。还检测了丙酮酸代谢的酶。发现苜蓿根瘤菌中L-阿拉伯糖的降解与已知的日本根瘤菌途径不同，因为前者缺乏2-酮基-o-脱氧-L-阿拉伯糖酸醛缩酶，但含有α-酮戊二酸半醛脱氢酶；因此，苜蓿根瘤菌可能具有导致α-酮戊二酸的L-阿拉伯糖途径，而不是导致乙醇醛和丙酮酸的途径。这一发现解释了突变体对L-阿拉伯糖呈阴性反应的原因。突变体的静止细胞能够代谢三种不支持生长的底物。
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