Cohen P L, Eisenberg R A
J Immunol. 1982 Nov;129(5):2142-5.
The role of T cell immunity against nuclear antigens recognized by autoantibodies in human and murine systemic lupus is unclear. We have studied the T cell proliferative response to purified Sm nuclear antigen by measuring in vitro 3H-thymidine incorporation by MRL/Mp- +/+ lymph node cells primed with Sm in Freund's adjuvant. Sm-primed cells responded in a dose-dependent fashion when cultured in vitro with purified Sm. Absorption of the Sm with solid-phase anti-Sm IgG from either of two lupus patients ablated the response, whereas control absorptions with normal IgG had no effect. Furthermore, cells from mice primed with physicochemically purified Sm responded in vitro to affinity-purified antigen, and vice versa. Treatment of cells with either anti-Thy-1 or anti-Lyt-1 and C eliminated Sm-induced proliferation, whereas anti-Lyt-2 treatment resulted in enhanced responses. MRL/Mp- +/+ mice, which made no detectable anti-Sm antibodies in vivo, were nonetheless capable of generating Sm-reactive T cells. These results indicate that T cells can specifically recognize the Sm nuclear antigen. Such recognition may be of importance in the generation of antibodies to Sm and other nuclear antigens in vivo.
在人类和小鼠系统性红斑狼疮中，针对自身抗体所识别的核抗原的T细胞免疫作用尚不清楚。我们通过测量用弗氏佐剂中Sm致敏的MRL/Mp- +/+淋巴结细胞在体外掺入3H-胸腺嘧啶核苷的量，研究了T细胞对纯化的Sm核抗原的增殖反应。当用纯化的Sm在体外培养时，经Sm致敏的细胞呈现剂量依赖性反应。用两名狼疮患者的固相抗Sm IgG吸收Sm可消除该反应，而用正常IgG进行对照吸收则无影响。此外，用物理化学方法纯化的Sm致敏的小鼠细胞在体外对亲和纯化的抗原产生反应，反之亦然。用抗Thy-1或抗Lyt-1及补体C处理细胞可消除Sm诱导的增殖，而抗Lyt-2处理则导致反应增强。体内未检测到抗Sm抗体的MRL/Mp- +/+小鼠仍能够产生对Sm有反应的T细胞。这些结果表明T细胞能够特异性识别Sm核抗原。这种识别在体内产生针对Sm和其他核抗原的抗体过程中可能具有重要意义。
