George C R
Neurochem Res. 1982 Feb;7(2):205-11. doi: 10.1007/BF00965058.
Alpha adrenergic receptor subtypes in rat hippocampal membranes were studied, using [3H] clonidine as the radioactive ligand. On the basis of competitive binding studies, using the selective antagonist-prazosin, WB-4101, and yohimbine, [3H] clonidine appeared to bind to a population of presynaptic sites that are pharmacologically similar to receptors previously classified as alpha 2. A computerized model that linearized and produced the best possible fit to the experimental data points indicated that [3H] clonidine binds to a single population of receptors possessing equal affinity for the ligand. Binding data also indicated that rat hippo-campus contains significantly fewer [3H]clonidine binding sites than rat cortex.
以[3H]可乐定为放射性配体，对大鼠海马膜中的α肾上腺素能受体亚型进行了研究。基于使用选择性拮抗剂哌唑嗪、WB - 4101和育亨宾的竞争性结合研究，[3H]可乐定似乎与一群药理学上类似于先前分类为α2的受体的突触前位点结合。一个将实验数据点线性化并产生最佳拟合的计算机模型表明，[3H]可乐定与对该配体具有同等亲和力的单一受体群体结合。结合数据还表明，大鼠海马中[3H]可乐定结合位点的数量明显少于大鼠皮质。
