Döhner L, Herrmann R M, Blinova V K, Ivanova V T
Acta Virol. 1982 Jul;26(4):227-33.
Two groups of antigenic recombinants Hav4N1 were obtained by recombination of human influenza virus H0N1 with two avian influenza viruses isolated from ducks in 1956 and terns in 1978 and possessing the same surface antigen Hav4 Nav1. Recombinants obtained by crossing A/PR/8/34 and A/duck/CSSR/56 viruses showed a lower ability to reproduce at optimal and lowered temperatures and differed in the thermosensitivity of haemagglutinin and neuraminidase. An analysis of virus-specific proteins of the recombinants revealed different combinations of genes coding for internal (Pl, NP) and nonstructural (NS1) proteins. Recombinants obtained by crossing A/PR/8/34 and A/tern/Frunze/334/78 viruses possessed a thermostable haemagglutinin; they produced plaques of a size characteristic of avian influenza but, as distinct from the latter, they were practically not eluted from fowl erythrocytes. Polypeptide analysis of these recombinants showed that the genes coding for NP, M and NS1 proteins were inherited from the A/tern/Frunze/334/78 strain.
通过将人流感病毒H0N1与1956年从鸭子中分离出的两种禽流感病毒以及1978年从燕鸥中分离出的两种禽流感病毒进行重组，获得了两组抗原重组体Hav4N1，这些禽流感病毒具有相同的表面抗原Hav4 Nav1。通过将A/PR/8/34和A/duck/CSSR/56病毒杂交获得的重组体在最佳温度和较低温度下繁殖的能力较低，并且血凝素和神经氨酸酶的热敏感性也有所不同。对重组体病毒特异性蛋白的分析揭示了编码内部（P1、NP）和非结构（NS1）蛋白的基因的不同组合。通过将A/PR/8/34和A/tern/Frunze/334/78病毒杂交获得的重组体具有耐热的血凝素；它们产生的蚀斑大小具有禽流感的特征，但与后者不同的是，它们实际上不会从禽红细胞中洗脱出来。对这些重组体的多肽分析表明，编码NP、M和NS1蛋白的基因是从A/tern/Frunze/334/78毒株遗传而来的。
