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成年雌性北美豹蛙(Rana pipiens)组织中单胺氧化酶活性的一些特征。

Some characteristics of monoamine oxidase activity in tissues of the adult female grassfrog (Rana pipiens).

作者信息

Urueña G, Hall T R

出版信息

Comp Biochem Physiol C Comp Pharmacol. 1982;73(1):67-70. doi: 10.1016/0306-4492(82)90169-1.

DOI:10.1016/0306-4492(82)90169-1
PMID:6128181
Abstract
  1. Monoamine oxidase (MAO) activity was measured fluorometrically in tissues of the grass-frog, Rana pipiens. 2. Incubation with specific inhibitors revealed the presence of two forms of MAO, type A and type B, in all tissues examined, though the ratios were different in each tissue. 3. Liver contained the greatest, and oviduct and skeletal muscle the lowest amounts of MAO activity, and brain also contained relatively high MAO levels. 4. The apparent Michaelis constant (Km) was similar in most tissues but was higher in pancreas, muscle and oviduct. Values of Km were lower than those reported for mammalian tissues.
摘要
  1. 采用荧光法测定了草蛙(豹蛙)组织中的单胺氧化酶(MAO)活性。2. 与特异性抑制剂共同孵育后发现,在所检测的所有组织中均存在两种形式的MAO,即A型和B型,不过每种组织中的比例有所不同。3. 肝脏中的MAO活性最高,输卵管和骨骼肌中的MAO活性最低,大脑中的MAO水平也相对较高。4. 大多数组织中的表观米氏常数(Km)相似,但胰腺、肌肉和输卵管中的Km较高。Km值低于哺乳动物组织的报道值。

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Comp Biochem Physiol C Comp Pharmacol. 1982;73(1):67-70. doi: 10.1016/0306-4492(82)90169-1.
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