Anniko M
Laryngol Rhinol Otol (Stuttg). 1982 Sep;61(9):534-4.
The in vitro behaviour of 51 consecutively operated cases was analyzed. Clinically, 18 tumours secreted GH, 15 had a concomitant secretion of GH and PRL, 10 secreted PRL, 3 secreted ACTH and 5 were clinically regarded as non-secreting. When using the organ culture technique with tissue in organotypic differentiation, hormone secretion (GH, PRL and ACTH) occurred during the whole 6 week period. Hormone secretion was correlated to weight in 74 specimens. A secretion rate of between 5000 and 55 000 pmol/mg wet weight/24 h of GH and 2500-7800 micrograms/mg wet weight/24 h of PRL occurred. Pituitary tumours clinically regarded as non-secreting showed a low secretion of GH (6-100 pmol/24 h/culture dish) and PRL (6,3-40 micrograms/24 h/culture dish). However, the hormone secretion rate in explants from these tumours rapidly decreased with time in vitro. No spontaneous degeneration of GH or PRL occurred during 2 h in vitro when the explant had been removed. In vivo irradiated tumours revealed a low secretion rate of GH, PRL and ACTH, respectively, when explanted to organ culture.
分析了51例连续手术病例的体外行为。临床上，18例肿瘤分泌生长激素（GH），15例同时分泌GH和催乳素（PRL），10例分泌PRL，3例分泌促肾上腺皮质激素（ACTH），5例临床上被视为无分泌功能。当采用器官培养技术使组织进行器官型分化时，在整个6周期间均出现了激素分泌（GH、PRL和ACTH）。74个标本的激素分泌与重量相关。GH的分泌率为5000至55000皮摩尔/毫克湿重/24小时，PRL的分泌率为2500至7800微克/毫克湿重/24小时。临床上被视为无分泌功能的垂体肿瘤显示GH分泌量低（6至100皮摩尔/24小时/培养皿），PRL分泌量低（6.3至40微克/24小时/培养皿）。然而，这些肿瘤外植体的激素分泌率在体外随时间迅速下降。当取出外植体后，在体外2小时内未发生GH或PRL的自发降解。体内照射过的肿瘤在移植到器官培养时，分别显示出低水平的GH、PRL和ACTH分泌率。
