Anniko M, Bagger-Sjöbäck D, Hultborn R
Arch Otorhinolaryngol. 1982;236(3):281-93. doi: 10.1007/BF00454220.
Studies on the respiration rate of human pituitary tumor tissue have so far been lacking in the literature. This study presents the results from four adenomas causing acromegaly, all with different clinical degrees of the disease. Determination of oxygen uptake was performed in vitro with a spectrophotorespirometric system. Pieces of the tumors were explanted to an organ culture system with a high degree of stability. The secretion rate of growth hormone (GH) and prolactin (PRL) was determined. After 4-8 days in vitro, specimens were analyzed for respiration rate. This was approximately 1-1.5 microliters O2/h/micrograms dry weight. The activity of the pituitary tumor tissue was characterized by both the hormone secretion rate and the respiration rate. Particularly active foci were found to occur in the adenoma tissue. Depending on the individual tumor, the GH secretion rate was approximately 0.1-100 pmol/micrograms dry weight/h and PRL secretion rate approximately 0.4-18 micrograms/micrograms dry weight/h. The respiration rate--as is also the hormone secretion rate--is dependent on the time in vitro prior to analysis. The respiration rate in individual tumors is a parameter which does not reflect GH or PRL serum levels or clinical activity of the disease.
迄今为止，文献中缺乏关于人类垂体肿瘤组织呼吸速率的研究。本研究展示了来自4例导致肢端肥大症的腺瘤的结果，所有病例的疾病临床程度各不相同。使用分光光度呼吸测定系统在体外测定氧气摄取量。将肿瘤组织块移植到具有高度稳定性的器官培养系统中。测定生长激素（GH）和催乳素（PRL）的分泌率。体外培养4 - 8天后，分析标本的呼吸速率。其约为1 - 1.5微升O₂/小时/微克干重。垂体肿瘤组织的活性通过激素分泌率和呼吸速率来表征。在腺瘤组织中发现了特别活跃的病灶。根据个体肿瘤情况，GH分泌率约为0.1 - 100皮摩尔/微克干重/小时，PRL分泌率约为0.4 - 18微克/微克干重/小时。呼吸速率——与激素分泌率一样——取决于分析前的体外培养时间。个体肿瘤中的呼吸速率是一个不反映GH或PRL血清水平或疾病临床活性的参数。
