Krizanová O, Veber P
Acta Virol. 1982 Sep;26(5):328-32.
The relationship between the density of chick embryo cells in stationary cultures and their specific 3',5'-cAMP phosphodiesterase (PDE) activities and calmodulin (CaM) contents were investigated. An indirect relationship between them was found. The specific CaM-PDE activities were the highest in the starting logarithmic phase of growth and the lowest in grown (72 hr old) monolayer cultures. Changes in the specific activities induced by two serum concentrations were also related to the cell culture density. The results suggested that the density or any metabolic change due to it is the primary factor regulating the level of specific PDE activities in chick embryo cells.
研究了静止培养的鸡胚细胞密度与其特异性3',5'-环磷酸腺苷磷酸二酯酶（PDE）活性和钙调蛋白（CaM）含量之间的关系。发现它们之间存在间接关系。特异性CaM-PDE活性在生长的起始对数期最高，而在生长（72小时龄）的单层培养物中最低。两种血清浓度诱导的比活性变化也与细胞培养密度有关。结果表明，密度或因其引起的任何代谢变化是调节鸡胚细胞中特异性PDE活性水平的主要因素。
