Bowden G, Mothibeli M A, Robb F T, Woods D R
J Gen Microbiol. 1982 Sep;128(9):2041-5. doi: 10.1099/00221287-128-9-2041.
The production of alkaline protease, collagenase and histidine utilization (Hut) enzymes by Vibrio alginolyticus wild-type, hutH1 and hutU1 strains was investigated. Alkaline protease synthesis was stimulated by histidine and urocanic acid in the wild-type and hutU1 strains. The hutH1 mutant alkaline protease production was stimulated by urocanic acid and not by histidine. The Hut enzymes in the wild-type strain were coordinately induced by histidine. Urocanase and formimino-hydrolase were induced by histidine in the hutH1 mutant which lacked histidase and was not able to convert histidine to urocanic acid. Collagenase production in peptone medium was inhibited in the hut mutants. It is concluded that in V. alginolyticus urocanic acid regulates alkaline protease synthesis but that the Hut enzymes are induced by histidine. The involvement of the Hut genetic system in the regulation of alkaline protease and collagenase synthesis is discussed.
研究了溶藻弧菌野生型、hutH1和hutU1菌株碱性蛋白酶、胶原酶和组氨酸利用（Hut）酶的产生情况。在野生型和hutU1菌株中，组氨酸和尿刊酸刺激碱性蛋白酶的合成。hutH1突变体的碱性蛋白酶产生受尿刊酸刺激，不受组氨酸刺激。野生型菌株中的Hut酶由组氨酸协同诱导。在缺乏组氨酸酶且无法将组氨酸转化为尿刊酸的hutH1突变体中，尿刊酸酶和亚胺甲基水解酶由组氨酸诱导。在蛋白胨培养基中，hut突变体的胶原酶产生受到抑制。得出的结论是，在溶藻弧菌中，尿刊酸调节碱性蛋白酶的合成，但Hut酶由组氨酸诱导。讨论了Hut遗传系统在碱性蛋白酶和胶原酶合成调控中的作用。
