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一种定量高密度递质识别终末密度和抗原含量的新方法。对大鼠尾状核壳核中不同类型酪氨酸羟化酶免疫反应性神经终末的免疫细胞化学研究。

A new approach to quantitate the density and antigen contents of high densities of transmitter-identified terminals. Immunocytochemical studies on different types of tyrosine hydroxylase immunoreactive nerve terminals in nucleus caudatus putamen of the rat.

作者信息

Agnati L F, Fuxe K, Zini I, Calza L, Benfenati F, Zoli M, Hökfelt T, Goldstein M

出版信息

Neurosci Lett. 1982 Oct 23;32(3):253-8. doi: 10.1016/0304-3940(82)90302-0.

DOI:10.1016/0304-3940(82)90302-0
PMID:6129602
Abstract

By means of a semi-automatic image analyzer plugged into an Apple II computer and suitable computer programs it is possible to analyze transmitter-identified nerve terminals. Thus, a densitometric approach is applied on the original photograph followed by systematic sampling which is carried out by means of a grating of circles. This procedure allows the study quantitatively of density and intensities of different types of densely packed tyrosine hydroxylase (TH) immunoreactive nerve terminals of the nuc. caudatus putamen. It is shown that the islandic TH immunoreactive nerve terminals have a higher density, but a TH content similar to the diffuse types of TH immunoreactive nerve terminals in the nuc. caudatus putamen.

摘要

通过连接到苹果II型计算机的半自动图像分析仪和合适的计算机程序,可以分析已识别递质的神经末梢。因此,先对原始照片采用密度测定法,然后通过圆形光栅进行系统采样。该程序能够定量研究尾壳核中不同类型的密集排列的酪氨酸羟化酶(TH)免疫反应性神经末梢的密度和强度。结果表明,岛状TH免疫反应性神经末梢密度较高,但TH含量与尾壳核中弥漫型TH免疫反应性神经末梢相似。

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Quantitative immunofluorescence of tyrosine hydroxylase in the adrenal medulla of spontaneously hypertensive rats.自发性高血压大鼠肾上腺髓质中酪氨酸羟化酶的定量免疫荧光分析
Experientia. 1985 Aug 15;41(8):1054-5. doi: 10.1007/BF01952140.