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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Scapin S, Autuori F, Baldini P, Incerpi S, Luly P, Sartori C

Comp Biochem Physiol B. 1982;73(4):779-83. doi: 10.1016/0305-0491(82)90317-0.

A subcellular fractionation procedure for frog liver is reported and validated by the distribution pattern of several marker enzymes, also in comparison with rat liver. 2. The subcellular distribution of tyrosine aminotransferase was investigated in frog liver as compared to rat liver: a different distribution of the enzyme was observed, being the activity mostly recovered in mitochondrial and cytosolic compartments. 3. Results indicate that mitochondrial tyrosine aminotransferase of both frog and rat liver is a matrix enzyme, even if differences are observed concerning its release from the organelles upon detergent treatment.

本文报道了一种青蛙肝脏的亚细胞分级分离方法，并通过几种标记酶的分布模式进行了验证，同时也与大鼠肝脏进行了比较。2. 研究了青蛙肝脏与大鼠肝脏中酪氨酸转氨酶的亚细胞分布：观察到该酶的分布不同，其活性主要在线粒体和胞质部分中检测到。3. 结果表明，青蛙和大鼠肝脏的线粒体酪氨酸转氨酶都是基质酶，尽管在去污剂处理后从细胞器释放方面存在差异。

Scapin S, Autuori F, Baldini P, Incerpi S, Luly P, Sartori C

Comp Biochem Physiol B. 1982;73(4):779-83. doi: 10.1016/0305-0491(82)90317-0.

A subcellular fractionation procedure for frog liver is reported and validated by the distribution pattern of several marker enzymes, also in comparison with rat liver. 2. The subcellular distribution of tyrosine aminotransferase was investigated in frog liver as compared to rat liver: a different distribution of the enzyme was observed, being the activity mostly recovered in mitochondrial and cytosolic compartments. 3. Results indicate that mitochondrial tyrosine aminotransferase of both frog and rat liver is a matrix enzyme, even if differences are observed concerning its release from the organelles upon detergent treatment.

本文报道了一种青蛙肝脏的亚细胞分级分离方法，并通过几种标记酶的分布模式进行了验证，同时也与大鼠肝脏进行了比较。2. 研究了青蛙肝脏与大鼠肝脏中酪氨酸转氨酶的亚细胞分布：观察到该酶的分布不同，其活性主要在线粒体和胞质部分中检测到。3. 结果表明，青蛙和大鼠肝脏的线粒体酪氨酸转氨酶都是基质酶，尽管在去污剂处理后从细胞器释放方面存在差异。