大鼠脑不同区域突触体组分及突触体膜的Ca2+ Mg2+ -ATP酶活性

Ca2+ Mg2+ -ATPase activity of synaptosome fraction and synaptosomal membranes from different areas of rat brain.

Lachowicz L, Janiszewska G, Wojtkowiak R, Wojtkowiak Z

Int J Biochem. 1983;15(2):163-5. doi: 10.1016/0020-711x(83)90061-7.

Abstract

Crude synaptosomal fractions obtained from four areas of rat brain were studied; cerebral cortex, hippocampus, midbrain, thalamus with hypothalamus, using the Cotman & Matthews method (1971) Biochim, biophys. Acta 249, 350-394. 2. The purity of synaptosomal fractions was controlled by electron microscopy, and by determination of some marker enzymes such as: LDH, MAO, AChE and cytochrome-c oxidase. 3. Synaptosomes were disrupted by hypoosmotical shock. 4. Crude synaptosomal membrane preparations indicated on increased Ca2+ Mg2+ -ATPase activity in comparison to the activity of this enzyme in synaptosomal fractions. 5. The incubation of crude synaptosomal membranes with cAMP and theophylline caused the subsequent increase of Ca2+ Mg2+ -ATPase activity, but mainly, in hippocampal region.

摘要

采用科特曼和马修斯方法（1971年，《生物化学与生物物理学学报》249卷，350 - 394页），对从大鼠脑四个区域获取的粗制突触体组分进行了研究，这四个区域分别是大脑皮层、海马体、中脑、丘脑与下丘脑。2. 通过电子显微镜以及对一些标记酶（如乳酸脱氢酶、单胺氧化酶、乙酰胆碱酯酶和细胞色素c氧化酶）的测定来控制突触体组分的纯度。3. 通过低渗休克破坏突触体。4. 与突触体组分中该酶的活性相比，粗制突触体膜制剂显示出Ca2+ Mg2+ -ATP酶活性增加。5. 粗制突触体膜与环磷酸腺苷和茶碱一起孵育会导致Ca2+ Mg2+ -ATP酶活性随后增加，但主要是在海马体区域。