Shigematsu H, Okamura N, Shimeno H, Kishimoto Y, Kan L, Fenselau C
J Neurochem. 1983 Mar;40(3):814-20. doi: 10.1111/j.1471-4159.1983.tb08052.x.
The conversion of lignoceric acid to cerebronic acid, ceramides, cerebrosides, and glutamic acid is catalyzed by a rat brain particulate preparation. The heat-stable factor, prepared from calf cerebellum, together with the heat-labile factor, a pyridine nucleotide, and Mg2+ are essential to all of these metabolic pathways. Our previous work showed that the heat-stable factor is composed of at least two components, HSF-1 and HSF-2, and identified HSF-2 as D-glucose-6-phosphate. In the current investigation, HSF-1 was further purified and found to be N-acetyl-L-aspartic acid. In addition, it was discovered that a third component, HSF-3, is also required for heat-stable factor activity. A reconstituted system composed of N-acetylaspartic acid, glucose-6-phosphate, and HSF-3 fully replaced the heat-stable factor essential for the conversion of lignoceric acid to cerebronic acid and glutamic acid. The reconstituted heat-stable factor did not show the initial time lag always observed with the crude heat-stable factor.
大鼠脑微粒体制剂可催化二十四烷酸转化为脑羟脂酸、神经酰胺、脑苷脂和谷氨酸。从小牛小脑制备的热稳定因子，与热不稳定因子（一种吡啶核苷酸）和Mg2+一起，对所有这些代谢途径都是必不可少的。我们之前的研究表明，热稳定因子至少由两种成分组成，即HSF-1和HSF-2，并确定HSF-2为D-葡萄糖-6-磷酸。在当前的研究中，HSF-1被进一步纯化，发现是N-乙酰-L-天冬氨酸。此外，还发现热稳定因子活性还需要第三种成分HSF-3。由N-乙酰天冬氨酸、葡萄糖-6-磷酸和HSF-3组成的重组系统完全替代了将二十四烷酸转化为脑羟脂酸和谷氨酸所必需的热稳定因子。重组的热稳定因子没有表现出粗制热稳定因子始终观察到的初始时间延迟。
