Fukuda H, Katsurada A, Iritani N
J Nutr Sci Vitaminol (Tokyo). 1982 Oct;28(5):527-32. doi: 10.3177/jnsv.28.527.
The effects of aging on enzyme qualities of glucose-6-phosphate dehydrogenase, malic enzyme and acetyl-CoA carboxylase were investigated. The Ouchterlony double-diffusion pattern showed that the enzymes from livers of rats of different ages are immunologically similar to each other. In addition, immunochemical titration demonstrated that the changes in the levels of activity of rats of different age are accompanied by proportionate changes in the quantity of the enzyme protein. The Kms of the enzymes for substrates were not altered by aging. It is suggested that the age-dependent impairments of the enzyme induction (Iritani, N., Fukuda, H., and Fukuda, E. (1981) Biochim. Biophys. Acta, 665, 639) can be ascribed to decreases in the rise of enzyme protein quantities.
研究了衰老对葡萄糖-6-磷酸脱氢酶、苹果酸酶和乙酰辅酶A羧化酶酶质量的影响。欧氏双扩散图谱显示，不同年龄大鼠肝脏中的酶在免疫上彼此相似。此外，免疫化学滴定表明，不同年龄大鼠活性水平的变化伴随着酶蛋白量的相应变化。衰老并未改变这些酶对底物的米氏常数。有人提出，酶诱导的年龄依赖性损伤（Iritani, N., Fukuda, H., and Fukuda, E. (1981) Biochim. Biophys. Acta, 665, 639）可归因于酶蛋白量增加的减少。
