Fukuda H, Iritani N
Biochim Biophys Acta. 1983 Oct 4;760(1):149-53. doi: 10.1016/0304-4165(83)90136-8.
The effects of insulin-treatment on induction of liver lipogenic enzymes by a fat-free diet were investigated in 1-, 2- and 9-month-old rats. The magnitudes of the induction of the enzymes were very high in 1-month-old rats and decreased with aging (Iritani, N., Fukuda, H. and Fukuda, E. (1981) Biochim. Biophys. Acta 665, 636). The induction of lipogenic enzymes was not affected by insulin injection in young rats. In 9-month-old rats, however, although the maximal magnitudes of the induction were not affected by the insulin injection, the lag periods of the induction were shortened. The lipogenic enzyme induction in 9-month-olds did not correlate with plasma insulin level but paralleled changes in the insulin binding capacities to liver plasma membranes and adipocytes. The results suggest that insulin binding is implicated in age-dependent decrease of lipogenic enzyme induction, and support our previous report (Iritani, N., Fukuda, H., Tashiro, S. and Ikeda, Y. (1982) J. Biochem. 92, 569).
在1月龄、2月龄和9月龄大鼠中研究了胰岛素治疗对无脂饮食诱导肝脏生脂酶的影响。这些酶的诱导幅度在1月龄大鼠中非常高，并随年龄增长而降低（入谷直、福田博、福田悦子（1981年）《生物化学与生物物理学学报》665卷，636页）。生脂酶的诱导在幼龄大鼠中不受胰岛素注射的影响。然而，在9月龄大鼠中，尽管诱导的最大幅度不受胰岛素注射的影响，但诱导的延迟期缩短。9月龄大鼠中生脂酶的诱导与血浆胰岛素水平无关，但与肝细胞膜和脂肪细胞的胰岛素结合能力的变化平行。结果表明，胰岛素结合与生脂酶诱导的年龄依赖性降低有关，并支持我们之前的报告（入谷直、福田博、田代史郎、池田洋（1982年）《生物化学杂志》92卷，569页）。
